Pulsed-Focused Ultrasound Enhances Boron Drug Accumulation in a Human Head and Neck Cancer Xenograft-Bearing Mouse Model
Abstract
Purpose
This study aims to demonstrate that pulsed high-intensity focused ultrasound (pulsed- HIFU) may enhance the fructose-conjugated 4-borono-L-phenylalanine (BPA-Fr) accumulation in tumor lesion using 18F-FBPA-Fr microPET scans.
Procedures
To the mice bearing orthotopic SASC03 human tongue squamous carcinoma xenograft, a 2-min pulsed-HIFU was applied to tumor. Immediately after pulsed-HIFU treatment, 18F-FBPA-Fr was intravenously injected, and biological characterizations including microPET imaging and biodistribution were conducted.
Results
Both biodistribution studies and microPET imaging performed after intravenous injection of 18F-FBPA-Fr revealed higher tumor uptake in HIFU-treated mice than that of the control. CD31 and Ki-67 histochemical staining of tumor sections and H&E staining of nearby normal tissues revealed no significant difference between the pulsed-HIFU-treated mice and the control.
Conclusion
This study demonstrated that pulsed-HIFU was beneficial to the accumulation of boron drug in the head and neck tumor lesion and may enhance the therapeutic efficacy of clinical BNCT.
Source: Hsin-Ell Wang
Chun-Yi Wu,1,2 Pei-Chia Chan,1 Lin-Shan Chou,1 Chi-Wei Chang,3 Feng-Yi Yang,1
Ren-Shyan Liu,3,4,5 Shih-Hwa Chiou,6 Yi-Wei Chen,7 Sang-Hue Yen,7 Hsin-Ell Wang1,2
(1)Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, No.155, Sec.2, Li-Nong St, 11217, Taipei, Taiwan
(2)Biophotonics & Molecular Imaging Research Center, National Yang-Ming University, Taipei, Taiwan
(3)Department of Nuclear Medicine and National PET/Cyclotron Center, Taipei Veterans General Hospital, Taipei, Taiwan
(4)Department of Nuclear Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan
(5)Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei, Taiwan
(6)Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan
(7)Cancer Center, Taipei Veterans General Hospital, Taipei, Taiwan